Plexus 2 Serial Number
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We performed this study with the following three blood collection methods: RP, RP with anesthesia (RA), and FV with anesthesia (FA). We compared the effects of three different methods on tissue injury, inflammation, and the amount of blood collected using the same blood volume (1 ml). All groups of mice were sacrificed at the end of the experiment, and blood was collected from the FV using the FV method, then from the RP using the RP and RA methods. The blood was centrifuged at 1,500 rpm for 10 min to collect serum for corticosterone analysis. The blood was then transferred to a tube containing clotting activator (ethylenediaminetetraacetic acid; EDTA) and centrifuged at 2,000 rpm for 15 min. After the blood was collected, the mice were sacrificed immediately. The eyes were examined for damage to surrounding tissues and the presence of inflammation in the RP, RA, and FV groups. The weight of the spleen and the number of immune cells in the peripheral blood and spleen were compared among the three groups using the Student t test. The numbers of the following immune cells were analyzed in the peripheral blood: neutrophils, basophils, eosinophils, lymphocytes, and monocytes. The number of each cell was determined using anti-mouse CD45 (Bio-Rad, CA, USA), anti-mouse Ly-6G/C, and anti-mouse CD11b monoclonal antibodies (mAbs) as described in the manufacturer’s manual. The number of each cell was determined by multiplying the percentage of each cell with the total number of leukocytes in the peripheral blood. The numbers of each cell in the spleen were analyzed using a fluorescence-activated cell sorter (FACS) Aria III (BD Biosciences, CA, USA).
RESULTS: The 25 EUS-CPB procedures were performed in 23 different patients. The mean age of the patients was 36.2 years (range 15-67 years). Male patients were predominant (12 patients), and the right brachial plexus was more frequently injured (15 lesions). The diagnosis was made by physical examination in 19 (76.0%) patients, and by imaging studies in 4 (16.0%) patients. The median duration from injury to EUS-CPB was 7.0 months (range 0-72 months). Using a visual analog scale (VAS) (0-100), median pain severity was significantly reduced from the preoperative period to the period of the 3-month follow-up after EUS-CPB (from 75.0 to 35.0, P 827ec27edc